WebThe 3.5-fold repression by TrpR in vivo disappeared when a tight LexA binding site was introduced at various sites near the trpO, suggesting that the binding of LexA blocks one-dimensional ... Web01. okt 2010. · A modular cassette, containing eight LexA-binding sites from the sulA operator and an hsp70 basal promoter, was cloned in pJFRC1 as a 5′-HindIII to 3′-BglII fragment yielding pJFRC18. Five LexA-binding sites were PCR amplified from pJFRC18-8XLexAop2-mCD8∷GFP with the inclusion of a 3′-SpeI restriction site.

Binding of the Bacillus subtilis LexA protein to the SOS operator

Web12. okt 2007. · LexA1 and LexA2 binding sites and their positions with respect to the ATG translational starting codon are indicated. The bent arrows indicate the positions of the oligonucleotides used to obtain the PP_3901 promoter probe for EMSA experiments. how to make notion background color

Burton Lexa 2024-2024 Women

Web03. sep 2015. · P1 is regulated by host LexA. (A) Genetic map of GIL01 with the P1–P2 promoter region enlarged below.Transcription start sites of the tandem promoters P1 and P2 are depicted with angled arrows (middle schematic) and shown in boldface letters (+1; bottom schematic). The −35 and −10 promoter consensus sequences are represented in … Web14. nov 2013. · (A) Sequence logo for LexA-binding sites in the Firmicutes (top) and in Bacillus subtilis (bottom). (B) Sequence logo for Fur-binding sites with experimental evidence of binding (top) or experimental evidence of regulation (bottom). Both examples are extracted from dynamically generated CollecTF reports and illustrate the ability to … Webthat consists of eight copies of the LexA binding sequence fused upstream of the −46 CaMV 35S minimal promoter. Olex-46 drives the expression of the Cre DNA recombinase derived from the P1 bacteriophage before it excises the sequence between the two loxP recognition sites. As a result, the GFP gene is located just downstream of the how to make notion page a template