Interaction analysis between OMD (or muteins) and collagen was analyzed using SPR in a Biacore T200 instrument (GE Healthcare). A CM5 Biacore sensor chip (GE Healthcare) was used and activated by a treatment with N-hydroxysuccinimide/N-ethyl-N′-(3-dimethylaminopropyl) carbodiimide hydrochloride, … Visa mer Expression and purification of human full-length OMD and muteins were performed as described previously11. Human OMD (without residues 1–20 corresponding to the signal peptide) with a C-terminal FLAG-tag was inserted into … Visa mer SEC-MALS was performed at room temperature and using PBS buffer (pH 7.4). Proteins concentrated at 25 µM were loaded on a Hiload 10/300 Superdex 200 pg column (GE … Visa mer Data collection was carried out at beamline AR-NE3A at the Photon Factory (Tsukuba, Japan) at 1.000 Å and 100 K. Diffraction images were processed with the program … Visa mer Purified OMD (9.9 mg/ml) was screened in an Oryx8 automatic protein crystallization system (Douglas Instrument) with commercially available … Visa mer Webb17 mars 2024 · However, their roles in fibrocartilage have not been widely reviewed. Here, we discuss both SLRP and large aggregating proteoglycan’s roles in collagen …
Collagen-binding by Drosophila SPARC is essential for survival and …
Webb15 aug. 2006 · This website requires cookies, and the limited processing of your personal data in order to function. By using the site you are agreeing to this as outlined in our … WebbDecorin and biglycan are two major small leucine-rich proteoglycans (SLRPs) present in the tendon extracellular matrix that facilitate collagen fibrillogenesis, tissue turnover, and … how are laws made in us
Collagen Structure & Function - Proteopedia, life in 3D
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